7WDU

6-sulfo-beta-D-N-acetylglucosaminidase from Bifidobacterium bifidum in complex with PUGNAc-6S

7WDU の概要

• エントリーDOI: 10.2210/pdb7wdu/pdb
• 分子名称: Beta-N-acetylhexosaminidase, [[(3R,4R,5S,6R)-3-acetamido-4,5-bis(oxidanyl)-6-(sulfooxymethyl)oxan-2-ylidene]amino] N-phenylcarbamate, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: glycoside hydrolase family 20, hydrolase
• 由来する生物種: Bifidobacterium bifidum JCM 1254
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 90685.12

構造登録者

Kashima, T.,Yamada, C.,Fushinobu, S.,Katoh, T.,Katayama, T. (登録日: 2021-12-22, 公開日: 2022-12-28, 最終更新日: 2024-11-06)

主引用文献

Katoh, T.,Yamada, C.,Wallace, M.D.,Yoshida, A.,Gotoh, A.,Arai, M.,Maeshibu, T.,Kashima, T.,Hagenbeek, A.,Ojima, M.N.,Takada, H.,Sakanaka, M.,Shimizu, H.,Nishiyama, K.,Ashida, H.,Hirose, J.,Suarez-Diez, M.,Nishiyama, M.,Kimura, I.,Stubbs, K.A.,Fushinobu, S.,Katayama, T.
A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem.
Nat.Chem.Biol., 19:778-789, 2023

PubMed Abstract: Mucinolytic bacteria modulate host-microbiota symbiosis and dysbiosis through their ability to degrade mucin O-glycans. However, how and to what extent bacterial enzymes are involved in the breakdown process remains poorly understood. Here we focus on a glycoside hydrolase family 20 sulfoglycosidase (BbhII) from Bifidobacterium bifidum, which releases N-acetylglucosamine-6-sulfate from sulfated mucins. Glycomic analysis showed that, in addition to sulfatases, sulfoglycosidases are involved in mucin O-glycan breakdown in vivo and that the released N-acetylglucosamine-6-sulfate potentially affects gut microbial metabolism, both of which were also supported by a metagenomic data mining analysis. Enzymatic and structural analysis of BbhII reveals the architecture underlying its specificity and the presence of a GlcNAc-6S-specific carbohydrate-binding module (CBM) 32 with a distinct sugar recognition mode that B. bifidum takes advantage of to degrade mucin O-glycans. Comparative analysis of the genomes of prominent mucinolytic bacteria also highlights a CBM-dependent O-glycan breakdown strategy used by B. bifidum.

PubMed: 36864192
DOI: 10.1038/s41589-023-01272-y

実験手法

X-RAY DIFFRACTION (2.23 Å)