7WCV
Co-crystal structure of FTO bound to 6e
Summary for 7WCV
| Entry DOI | 10.2210/pdb7wcv/pdb |
| Descriptor | Alpha-ketoglutarate-dependent dioxygenase FTO, 2-OXOGLUTARIC ACID, MANGANESE (II) ION, ... (5 entities in total) |
| Functional Keywords | rna demethylase, rna binding protein, oxidoreductase |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 57020.88 |
| Authors | Yang, C.-G.,Gan, J.H. (deposition date: 2021-12-20, release date: 2022-07-06, Last modification date: 2023-11-29) |
| Primary citation | Liu, Z.,Duan, Z.,Zhang, D.,Xiao, P.,Zhang, T.,Xu, H.,Wang, C.H.,Rao, G.W.,Gan, J.,Huang, Y.,Yang, C.G.,Dong, Z. Structure-Activity Relationships and Antileukemia Effects of the Tricyclic Benzoic Acid FTO Inhibitors. J.Med.Chem., 65:10638-10654, 2022 Cited by PubMed Abstract: The -methyladenosine (mA) demethylase FTO is overexpressed in acute myeloid leukemia (AML) cells and promotes leukemogenesis. We previously developed tricyclic benzoic acid FB23 as a highly potent FTO inhibitor in vitro. However, it showed a moderate antiproliferative effect on AML cells. In this work, we performed a structure-activity relationship study of tricyclic benzoic acids as FTO inhibitors. The analog exhibited excellent inhibitory effects on FTO similar to that of FB23 in vitro. In contrast to FB23, exerted a strong antiproliferative effect on AML cells. Like knock down, upregulated and expression and increased the protein abundance while it downregulated expression and decreased MYC protein abundance. These genes are key FTO targets in AML cells. Finally, treatment improved the survival rate of MONOMAC6-transplanted NSG mice. Collectively, our data suggest that targeting FTO with tricyclic benzoic acid inhibitors may be a potential strategy for treating AML. PubMed: 35793358DOI: 10.1021/acs.jmedchem.2c00848 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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