7W6D

Polyketide cyclase OAC-F24I mutant from Cannabis sativa in complex with olivetolic acid

7W6D の概要

• エントリーDOI: 10.2210/pdb7w6d/pdb
• 分子名称: Olivetolic acid cyclase, 2,4-bis(oxidanyl)-6-pentyl-benzoic acid (3 entities in total)
• 機能のキーワード: olivetolic acid cyclase, polypeptide cyclase, lyase
• 由来する生物種: Cannabis sativa (Hemp, Marijuana)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24840.50

構造登録者

Nakashima, Y.,Lee, Y.E.,Morita, H. (登録日: 2021-12-01, 公開日: 2022-02-02, 最終更新日: 2023-11-29)

主引用文献

Lee, Y.E.,Nakashima, Y.,Kodama, T.,Chen, X.,Morita, H.
Dual Engineering of Olivetolic Acid Cyclase and Tetraketide Synthase to Generate Longer Alkyl-Chain Olivetolic Acid Analogs.
Org.Lett., 24:410-414, 2022

PubMed Abstract: The therapeutic effects of Δ-tetrahydrocannabinol (Δ-THC) can be enhanced by modifications of the pentyl moiety at C-3. The engineering of olivetolic acid cyclase and tetraketide synthase with F24I and L190G substitutions, respectively, in the biosynthesis of Δ-THC serves as a platform for the generation of resorcylic acids up to 6-undecylresorcylic acid. These results provide insights into the development of THC analogs with chemically distinct acyl moieties at C-3.

PubMed: 34939812
DOI: 10.1021/acs.orglett.1c04089

実験手法

X-RAY DIFFRACTION (1.54 Å)