7W5A

The cryo-EM structure of human pre-C*-II complex

7W5A の概要

• エントリーDOI: 10.2210/pdb7w5a/pdb
• EMDBエントリー: 32319
• 分子名称: Pre-mRNA-processing-splicing factor 8, Crooked neck-like protein 1, Pre-mRNA-splicing factor SPF27, ... (49 entities in total)
• 機能のキーワード: rna splicing, prp22, exon ligation, human spliceosome, c* complex, splicing
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 54
• 化学式量合計: 2864582.10

構造登録者

Zhan, X.,Lu, Y.,Shi, Y. (登録日: 2021-11-29, 公開日: 2022-06-22, 最終更新日: 2025-07-02)

主引用文献

Zhan, X.,Lu, Y.,Zhang, X.,Yan, C.,Shi, Y.
Mechanism of exon ligation by human spliceosome.
Mol.Cell, 82:2769-2778.e4, 2022

PubMed Abstract: Pre-mRNA splicing involves two sequential reactions: branching and exon ligation. The C complex after branching undergoes remodeling to become the C complex, which executes exon ligation. Here, we report cryo-EM structures of two intermediate human spliceosomal complexes, pre-C-I and pre-C-II, both at 3.6 Å. In both structures, the 3' splice site is already docked into the active site, the ensuing 3' exon sequences are anchored on PRP8, and the step II factor FAM192A contacts the duplex between U2 snRNA and the branch site. In the transition of pre-C-I to pre-C-II, the step II factors Cactin, FAM32A, PRKRIP1, and SLU7 are recruited. Notably, the RNA helicase PRP22 is positioned quite differently in the pre-C-I, pre-C-II, and C complexes, suggesting a role in 3' exon binding and proofreading. Together with information on human C and C complexes, our studies recapitulate a molecular choreography of the C-to-C transition, revealing mechanistic insights into exon ligation.

PubMed: 35705093
DOI: 10.1016/j.molcel.2022.05.021

実験手法

ELECTRON MICROSCOPY (3.6 Å)