7VZG

Structure of the Acidobacteria homodimeric reaction center bound with cytochrome c (the larger form)

Summary for 7VZG

• Entry DOI: 10.2210/pdb7vzg/pdb
• EMDB information: 32228
• Descriptor: PscA, [methyl 9-acetyl-14-ethyl-20-hydroxy-4,8,13,18-tetramethyl-3-{3-oxo-3-[(3,7,11,15-tetramethylhexadec-2-en-1-yl)oxy]propyl}-3,4,20,21-tetradehydrophorbine-21-carboxylatato(2-)-kappa~4~N~23~,N~24~,N~25~,N~26~]zinc, BACTERIOCHLOROPHYLL A, ... (21 entities in total)
• Functional Keywords: membrane protein, photosynthesis
• Biological source: Chloracidobacterium thermophilum; More
• Total number of polymer chains: 14
• Total formula weight: 337002.72

Authors

Huang, G.Q.,Dong, S.S.,Qin, X.C.,Sui, S.F. (deposition date: 2021-11-16, release date: 2023-02-22)

Primary citation

Dong, S.,Huang, G.,Wang, C.,Wang, J.,Sui, S.F.,Qin, X.
Structure of the Acidobacteria homodimeric reaction center bound with cytochrome c.
Nat Commun, 13:7745-7745, 2022

PubMed Abstract: Photosynthesis converts light energy to chemical energy to fuel life on earth. Light energy is harvested by antenna pigments and transferred to reaction centers (RCs) to drive the electron transfer (ET) reactions. Here, we present cryo-electron microscopy (cryo-EM) structures of two forms of the RC from the microaerophilic Chloracidobacterium thermophilum (CabRC): one containing 10 subunits, including two different cytochromes; and the other possessing two additional subunits, PscB and PscZ. The larger form contained 2 Zn-bacteriochlorophylls, 16 bacteriochlorophylls, 10 chlorophylls, 2 lycopenes, 2 hemes, 3 FeS clusters, 12 lipids, 2 Ca ions and 6 water molecules, revealing a type I RC with an ET chain involving two hemes and a hybrid antenna containing bacteriochlorophylls and chlorophylls. Our results provide a structural basis for understanding the excitation energy and ET within the CabRC and offer evolutionary insights into the origin and adaptation of photosynthetic RCs.

PubMed: 36517472
DOI: 10.1038/s41467-022-35460-6

Experimental method

ELECTRON MICROSCOPY (2.61 Å)