7VS9

Crystal structure of P domain from norovirus GI.9 capsid protein in complex with Lewis x antigen.

7VS9 の概要

• エントリーDOI: 10.2210/pdb7vs9/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900120
• 分子名称: VP1, alpha-L-fucopyranose-(1-3)-[beta-D-galactopyranose-(1-4)]2-acetamido-2-deoxy-alpha-D-glucopyranose, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: norovirus, p-domain, capsid, histo blood group antigen, viral protein
• 由来する生物種: Norovirus Hu/GI/Vancouver730/2004/CAN
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69230.54

構造登録者

Murayama, K.,Kato-Murayama, M.,Shirouzu, M. (登録日: 2021-10-26, 公開日: 2022-08-31, 最終更新日: 2023-11-29)

主引用文献

Kimura-Someya, T.,Kato-Murayama, M.,Katsura, K.,Sakai, N.,Murayama, K.,Hanada, K.,Shirouzu, M.,Someya, Y.
Lewis fucose is a key moiety for the recognition of histo-blood group antigens by GI.9 norovirus, as revealed by structural analysis.
Febs Open Bio, 12:560-570, 2022

PubMed Abstract: Noroviruses have been identified as major causative agents of acute nonbacterial gastroenteritis in humans. Histo-blood group antigens (HBGAs) are thought to play a major role among the host cellular factors influencing norovirus infection. Genogroup I, genotype 9 (GI.9) is the most recently identified genotype within genogroup I, whose representative strain is the Vancouver 730 norovirus. However, the molecular interactions between host antigens and the GI.9 capsid protein have not been investigated in detail. In this study, we demonstrate that the GI.9 norovirus preferentially binds Lewis antigens over blood group A, B, and H antigens, as revealed by an HBGA binding assay using virus-like particles. We determined the crystal structures of the protruding domain of the GI.9 capsid protein in the presence or absence of Lewis antigens. Our analysis demonstrated that Lewis fucose (α1-3/4 fucose) represents a key moiety for the GI.9 protein-HBGA interaction, thus suggesting that Lewis antigens might play a critical role during norovirus infection. In addition to previously reported findings, our observations may support the future design of antiviral agents and vaccines against noroviruses.

PubMed: 35038379
DOI: 10.1002/2211-5463.13370

実験手法

X-RAY DIFFRACTION (2.26 Å)