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7VRU

Crystal structure of PacII_M1M2S-DNA-SAH complex

7VRU の概要
エントリーDOI10.2210/pdb7vru/pdb
分子名称Site-specific DNA-methyltransferase (adenine-specific), Site-specific DNA recognition subunit, DNA (25-mer), ... (7 entities in total)
機能のキーワードtype i r-m system pacii methytransferase m4c and m6a modification complex, biosynthetic protein, transferase-dna complex, transferase/dna
由来する生物種Pseudomonas alcaligenes
詳細
タンパク質・核酸の鎖数5
化学式量合計174329.84
構造登録者
Zhu, J.,Gao, P. (登録日: 2021-10-25, 公開日: 2022-11-30, 最終更新日: 2024-10-16)
主引用文献Zhu, J.,Gao, Y.,Wang, Y.,Zhan, Q.,Feng, H.,Luo, X.,Li, P.,Liu, S.,Hou, H.,Gao, P.
Molecular insights into DNA recognition and methylation by non-canonical type I restriction-modification systems.
Nat Commun, 13:6391-6391, 2022
Cited by
PubMed Abstract: Type I restriction-modification systems help establish the prokaryotic DNA methylation landscape and provide protection against invasive DNA. In addition to classical m6A modifications, non-canonical type I enzymes catalyze both m6A and m4C using alternative DNA-modification subunits M1 and M2. Here, we report the crystal structures of the non-canonical PacII_M1M2S methyltransferase bound to target DNA and reaction product S-adenosylhomocysteine in a closed clamp-like conformation. Target DNA binds tightly within the central tunnel of the M1M2S complex and forms extensive contacts with all three protein subunits. Unexpectedly, while the target cytosine properly inserts into M2's pocket, the target adenine (either unmethylated or methylated) is anchored outside M1's pocket. A unique asymmetric catalysis is established where PacII_M1M2S has precisely coordinated the relative conformations of different subunits and evolved specific amino acids within M2/M1. This work provides insights into mechanisms of m6A/m4C catalysis and guidance for designing tools based on type I restriction-modification enzymes.
PubMed: 36302770
DOI: 10.1038/s41467-022-34085-z
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.4 Å)
構造検証レポート
Validation report summary of 7vru
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-13に公開中

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