7VRG

Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B

7VRG の概要

• エントリーDOI: 10.2210/pdb7vrg/pdb
• 分子名称: Chitinase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, methyl 4-[5,6-bis(chloranyl)-1H-indol-3-yl]-3-(5-chloranyl-1H-indol-3-yl)-1H-pyrrole-2-carboxylate, ... (5 entities in total)
• 機能のキーワード: chitinase h, o. furnacalis, lynamicin b, hydrolase
• 由来する生物種: Ostrinia furnacalis (Asian corn borer)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 122183.17

構造登録者

Lu, Q.,Liu, T.,Zhou, Y.,Yang, Q. (登録日: 2021-10-22, 公開日: 2022-03-02, 最終更新日: 2024-10-30)

主引用文献

Lu, Q.,Xu, L.,Liu, L.,Zhou, Y.,Liu, T.,Song, Y.,Ju, J.,Yang, Q.
Lynamicin B is a Potential Pesticide by Acting as a Lepidoptera-Exclusive Chitinase Inhibitor.
J.Agric.Food Chem., 69:14086-14091, 2021

PubMed Abstract: Insect group h chitinase is a promising target for designing non-target safe pesticides in that it is exclusively distributed in lepidopteran insects, over 80% of which are agricultural pests. In this work, lynamicin B was discovered to be an inhibitor of Chi-h, the group h chitinase from the lepidopteran pest . Lynamicin B was revealed to competitively inhibit Chi-h with a value of 8.76 μM and does not significantly inhibit other chitinases. The co-crystal structure of lynamicin B and Chi-h revealed that the dichloroindolyl group of lynamicin B occupies an unexplored pocket below subsites +1 and +2 of the substrate-binding cleft, which is vital for its selectivity. Feeding experiments demonstrated that lynamicin B exhibited high insecticidal activities against other lepidopteran pests and besides . Moreover, lynamicin B did not affect , a natural enemy of . This study provides a natural-derived potent pesticide for the control of lepidopteran pests, leaving its natural enemy unaffected.

PubMed: 34797675
DOI: 10.1021/acs.jafc.1c05385

実験手法

X-RAY DIFFRACTION (2.4 Å)