7VOY
Rba sphaeroides PufX-KO RC-LH1
7VOY の概要
エントリーDOI | 10.2210/pdb7voy/pdb |
関連するPDBエントリー | 7VA9 7VB9 7VNM 7VNY |
EMDBエントリー | 32062 |
分子名称 | Light-harvesting protein B-875 alpha chain, SPEROIDENONE, Light-harvesting protein B-875 beta chain, ... (10 entities in total) |
機能のキーワード | photosystem, complex, photosynthesis, mutant |
由来する生物種 | Cereibacter sphaeroides 2.4.1 詳細 |
タンパク質・核酸の鎖数 | 37 |
化学式量合計 | 343811.76 |
構造登録者 | Bracun, L.,Yamagata, A.,Liu, L.N.,Shirouzu, M. (登録日: 2021-10-15, 公開日: 2022-05-04, 最終更新日: 2024-06-19) |
主引用文献 | Cao, P.,Bracun, L.,Yamagata, A.,Christianson, B.M.,Negami, T.,Zou, B.,Terada, T.,Canniffe, D.P.,Shirouzu, M.,Li, M.,Liu, L.N. Structural basis for the assembly and quinone transport mechanisms of the dimeric photosynthetic RC-LH1 supercomplex. Nat Commun, 13:1977-1977, 2022 Cited by PubMed Abstract: The reaction center (RC) and light-harvesting complex 1 (LH1) form a RC-LH1 core supercomplex that is vital for the primary reactions of photosynthesis in purple phototrophic bacteria. Some species possess the dimeric RC-LH1 complex with a transmembrane polypeptide PufX, representing the largest photosynthetic complex in anoxygenic phototrophs. However, the details of the architecture and assembly mechanism of the RC-LH1 dimer are unclear. Here we report seven cryo-electron microscopy (cryo-EM) structures of RC-LH1 supercomplexes from Rhodobacter sphaeroides. Our structures reveal that two PufX polypeptides are positioned in the center of the S-shaped RC-LH1 dimer, interlocking association between the components and mediating RC-LH1 dimerization. Moreover, we identify another transmembrane peptide, designated PufY, which is located between the RC and LH1 subunits near the LH1 opening. PufY binds a quinone molecule and prevents LH1 subunits from completely encircling the RC, creating a channel for quinone/quinol exchange. Genetic mutagenesis, cryo-EM structures, and computational simulations provide a mechanistic understanding of the assembly and electron transport pathways of the RC-LH1 dimer and elucidate the roles of individual components in ensuring the structural and functional integrity of the photosynthetic supercomplex. PubMed: 35418573DOI: 10.1038/s41467-022-29563-3 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (4.2 Å) |
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