7VOO

Induced alpha-2-macroglobulin monomer

Summary for 7VOO

• Entry DOI: 10.2210/pdb7voo/pdb
• EMDB information: 32052
• Descriptor: Alpha-2-macroglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: protease inhibitor, blood clotting
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 147766.67

Authors

Huang, X.,Wang, Y.,Ping, Z. (deposition date: 2021-10-14, release date: 2022-10-19, Last modification date: 2024-11-06)

Primary citation

Huang, X.,Wang, Y.,Yu, C.,Zhang, H.,Ru, Q.,Li, X.,Song, K.,Zhou, M.,Zhu, P.
Cryo-EM structures reveal the dynamic transformation of human alpha-2-macroglobulin working as a protease inhibitor.
Sci China Life Sci, 65:2491-2504, 2022

PubMed Abstract: Human alpha-2-macroglobulin is a well-known inhibitor of a broad spectrum of proteases and plays important roles in immunity, inflammation, and infections. Here, we report the cryo-EM structures of human alpha-2-macroglobulin in its native state, induced state transformed by its authentic substrate, human trypsin, and serial intermediate states between the native and fully induced states. These structures exhibit distinct conformations, which reveal the dynamic transformation of alpha-2-macro-globulin that acts as a protease inhibitor. The results shed light on the molecular mechanism of alpha-2-macroglobulin in entrapping substrates.

PubMed: 35781771
DOI: 10.1007/s11427-022-2139-2

Experimental method

ELECTRON MICROSCOPY (3.9 Å)