7VOO

Induced alpha-2-macroglobulin monomer

7VOO の概要

• エントリーDOI: 10.2210/pdb7voo/pdb
• EMDBエントリー: 32052
• 分子名称: Alpha-2-macroglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: protease inhibitor, blood clotting
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 147766.67

構造登録者

Huang, X.,Wang, Y.,Ping, Z. (登録日: 2021-10-14, 公開日: 2022-10-19, 最終更新日: 2024-11-06)

主引用文献

Huang, X.,Wang, Y.,Yu, C.,Zhang, H.,Ru, Q.,Li, X.,Song, K.,Zhou, M.,Zhu, P.
Cryo-EM structures reveal the dynamic transformation of human alpha-2-macroglobulin working as a protease inhibitor.
Sci China Life Sci, 65:2491-2504, 2022

PubMed Abstract: Human alpha-2-macroglobulin is a well-known inhibitor of a broad spectrum of proteases and plays important roles in immunity, inflammation, and infections. Here, we report the cryo-EM structures of human alpha-2-macroglobulin in its native state, induced state transformed by its authentic substrate, human trypsin, and serial intermediate states between the native and fully induced states. These structures exhibit distinct conformations, which reveal the dynamic transformation of alpha-2-macro-globulin that acts as a protease inhibitor. The results shed light on the molecular mechanism of alpha-2-macroglobulin in entrapping substrates.

PubMed: 35781771
DOI: 10.1007/s11427-022-2139-2

実験手法

ELECTRON MICROSCOPY (3.9 Å)