7VOK

The Crystal structure of EF-Tu and GDP from Mycobacterium tuberculosis

7VOK の概要

• エントリーDOI: 10.2210/pdb7vok/pdb
• 分子名称: Elongation factor Tu, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: mycobacterium tuberculosis, elongation factor tu, xray, elongation factor
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 176467.73

構造登録者

Zhan, B.W.,Li, J.X. (登録日: 2021-10-14, 公開日: 2022-10-19, 最終更新日: 2024-04-24)

主引用文献

Zhan, B.,Gao, Y.,Gao, W.,Li, Y.,Li, Z.,Qi, Q.,Lan, X.,Shen, H.,Gan, J.,Zhao, G.,Li, J.
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis.
Commun Biol, 5:1052-1052, 2022

PubMed Abstract: Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) is the second-deadliest infectious disease worldwide. Emerging evidence shows that the elongation factor EF-Tu could be an excellent target for treating Mtb infection. Here, we report the crystal structures of Mtb EF-Tu•EF-Ts and EF-Tu•GDP complexes, showing the molecular basis of EF-Tu's representative recycling and inactive forms in protein translation. Mtb EF-Tu binds with EF-Ts at a 1:1 ratio in solution and crystal packing. Mutation and SAXS analysis show that EF-Ts residues Arg13, Asn82, and His149 are indispensable for the EF-Tu/EF-Ts complex formation. The GDP binding pocket of EF-Tu dramatically changes conformations upon binding with EF-Ts, sharing a similar GDP-exchange mechanism in E. coli and T. ther. Also, the FDA-approved drug Osimertinib inhibits the growth of M. smegmatis, H37Ra, and M. bovis BCG strains by directly binding with EF-Tu. Thus, our work reveals the structural basis of Mtb EF-Tu in polypeptide synthesis and may provide a promising candidate for TB treatment.

PubMed: 36192483
DOI: 10.1038/s42003-022-04019-y

実験手法

X-RAY DIFFRACTION (3.4 Å)