7VMZ

Crystal structure of a human Coronavirus 229E antibody C04 Fab

7VMZ の概要

• エントリーDOI: 10.2210/pdb7vmz/pdb
• 分子名称: C04 Fab Light Chain, C04 Fab Heavy Chain (2 entities in total)
• 機能のキーワード: the antibody c04 targeted rbd of human coronavirus 229e spike, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47648.00

構造登録者

Xiang, J.C.,Yang, B. (登録日: 2021-10-10, 公開日: 2022-10-12, 最終更新日: 2024-11-13)

主引用文献

Xiang, J.,Su, J.,Lan, Q.,Zhao, W.,Zhou, Y.,Xu, Y.,Niu, J.,Xia, S.,Qi, Q.,Sidhu, S.,Lu, L.,Miersch, S.,Yang, B.
Antigenic mapping reveals sites of vulnerability on alpha-HCoV spike protein.
Commun Biol, 5:1179-1179, 2022

PubMed Abstract: Understanding the antigenic signatures of all human coronaviruses (HCoVs) Spike (S) proteins is imperative for pan-HCoV epitopes identification and broadly effective vaccine development. To depict the currently elusive antigenic signatures of α-HCoVs S proteins, we isolated a panel of antibodies against the HCoV-229E S protein and characterized their epitopes and neutralizing potential. We found that the N-terminal domain of HCoV-229E S protein is antigenically dominant wherein an antigenic supersite is present and appears conserved in HCoV-NL63, which holds potential to serve as a pan-α-HCoVs epitope. In the receptor binding domain, a neutralizing epitope is captured in the end distal to the receptor binding site, reminiscent of the locations of the SARS-CoV-2 RBD cryptic epitopes. We also identified a neutralizing antibody that recognizes the connector domain, thus representing the first S2-directed neutralizing antibody against α-HCoVs. The unraveled HCoVs S proteins antigenic similarities and variances among genera highlight the challenges faced by pan-HCoV vaccine design while supporting the feasibility of broadly effective vaccine development against a subset of HCoVs.

PubMed: 36333470
DOI: 10.1038/s42003-022-04160-8

実験手法

X-RAY DIFFRACTION (2.85 Å)