7VM8

Crystal structure of the MtDMI1 gating ring

7VM8 の概要

• エントリーDOI: 10.2210/pdb7vm8/pdb
• 分子名称: Ion channel DMI1 (1 entity in total)
• 機能のキーワード: dmi, symbiose, plant protein
• 由来する生物種: Medicago truncatula (Barrel medic, Medicago tribuloides)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60475.39

構造登録者

Huang, X.,Zhang, P. (登録日: 2021-10-08, 公開日: 2022-08-24, 最終更新日: 2023-11-29)

主引用文献

Liu, H.,Lin, J.S.,Luo, Z.,Sun, J.,Huang, X.,Yang, Y.,Xu, J.,Wang, Y.F.,Zhang, P.,Oldroyd, G.E.D.,Xie, F.
Constitutive activation of a nuclear-localized calcium channel complex in Medicago truncatula.
Proc.Natl.Acad.Sci.USA, 119:e2205920119-e2205920119, 2022

PubMed Abstract: Nuclear Ca oscillations allow symbiosis signaling, facilitating plant recognition of beneficial microsymbionts, nitrogen-fixing rhizobia, and nutrient-capturing arbuscular mycorrhizal fungi. Two classes of channels, DMI1 and CNGC15, in a complex on the nuclear membrane, coordinate symbiotic Ca oscillations. However, the mechanism of Ca signature generation is unknown. Here, we demonstrate spontaneous activation of this channel complex, through gain-of-function mutations in , leading to spontaneous nuclear Ca oscillations and spontaneous nodulation, in a -dependent manner. The mutations destabilize a hydrogen-bond or salt-bridge network between two RCK domains, with the resultant structural changes, alongside DMI1 cation permeability, activating the channel complex. This channel complex was reconstituted in human HEK293T cell lines, with the resultant calcium influx enhanced by autoactivated DMI1 and CNGC15s. Our results demonstrate the mode of activation of this nuclear channel complex, show that DMI1 and CNGC15 are sufficient to create oscillatory Ca signals, and provide insights into its native mode of induction.

PubMed: 35972963
DOI: 10.1073/pnas.2205920119

実験手法

X-RAY DIFFRACTION (3.034 Å)