7VI8

Crystal structure of ChbG

7VI8 の概要

• エントリーDOI: 10.2210/pdb7vi8/pdb
• 分子名称: Chitooligosaccharide deacetylase, ZINC ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: chbg, chitin catabolism, chitin deacetylase, chitobiose operon, klebsiella pneumoniae, hydrolase
• 由来する生物種: Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56109.02

構造登録者

Lee, S.Y.,Park, H.H. (登録日: 2021-09-26, 公開日: 2022-09-07, 最終更新日: 2023-11-29)

主引用文献

Lee, S.Y.,Pardhe, B.D.,Oh, T.J.,Park, H.H.
Crystal structure of ChbG from Klebsiella pneumoniae reveals the molecular basis of diacetylchitobiose deacetylation.
Commun Biol, 5:862-862, 2022

PubMed Abstract: The chitobiose (chb) operon is involved in the synthesis of chitooligosaccharide and is comprised of a BCARFG gene cluster. ChbG encodes a chitooligosaccharide deacetylase (CDA) which catalyzes the removal of one acetyl group from N,N'-diacetylchitobiose. It is considered a novel type of CDA due to its lack of sequence homology. Although there are various structural studies of CDAs linked to the kinetic properties of the enzyme, the structural information of ChbG is unavailable. In this study, the crystal structure of ChbG from Klebsiella pneumoniae is provided. The molecular basis of deacetylation of diacetylchitobiose by ChbG is determined based on structural analysis, mutagenesis, biophysical analysis, and in silico docking of the substrate, diacetylchitobiose. This study contributes towards a deeper understanding of chitin and chitosan biology, as well as provides a platform to engineer CDA biocatalysts.

PubMed: 36002585
DOI: 10.1038/s42003-022-03824-9

実験手法

X-RAY DIFFRACTION (1.83 Å)