7VG2

Cryo-EM structure of Arabidopsis DCL3 in complex with a 40-bp RNA

7VG2 の概要

• エントリーDOI: 10.2210/pdb7vg2/pdb
• EMDBエントリー: 31963
• 分子名称: Dicer-like 3, TAS1a forward strand (5'-phosphorylation), TAS1a reverse strand, ... (5 entities in total)
• 機能のキーワード: dcl3, rna, rddm, dicer, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 208177.70

構造登録者

Wang, Q.,Du, J. (登録日: 2021-09-14, 公開日: 2021-10-27, 最終更新日: 2024-06-19)

主引用文献

Wang, Q.,Xue, Y.,Zhang, L.,Zhong, Z.,Feng, S.,Wang, C.,Xiao, L.,Yang, Z.,Harris, C.J.,Wu, Z.,Zhai, J.,Yang, M.,Li, S.,Jacobsen, S.E.,Du, J.
Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation.
Science, 374:1152-1157, 2021

PubMed Abstract: In eukaryotes, small RNAs (sRNAs) play critical roles in multiple biological processes. Dicer endonucleases are a central part of sRNA biogenesis. In plants, DICER-LIKE PROTEIN 3 (DCL3) produces 24-nucleotide (nt) small interfering RNAs (siRNAs) that determine the specificity of the RNA-directed DNA methylation pathway. Here, we determined the structure of a DCL3–pre-siRNA complex in an active dicing-competent state. The 5′-phosphorylated A1 of the guide strand and the 1-nt 3′ overhang of the complementary strand are specifically recognized by a positively charged pocket and an aromatic cap, respectively. The 24-nt siRNA length dependence relies on the separation between the 5′-phosphorylated end of the guide RNA and dual cleavage sites formed by the paired ribonuclease III domains. These structural studies, complemented by functional data, provide insight into the dicing principle for Dicers in general.

PubMed: 34648373
DOI: 10.1126/science.abl4546

実験手法

ELECTRON MICROSCOPY (3.1 Å)