7VF6

The crystal structure of PurZ0

7VF6 の概要

• エントリーDOI: 10.2210/pdb7vf6/pdb
• 分子名称: PurA-like adenylosuccinate synthetase, PHOSPHATE ION, GUANOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: pura-like adenylosuccinate synthetase, ligase
• 由来する生物種: Gordonia phage Archimedes
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82528.99

構造登録者

Tong, Y.,Zhang, Y. (登録日: 2021-09-10, 公開日: 2023-05-24, 最終更新日: 2024-04-03)

主引用文献

Tong, Y.,Wu, X.,Liu, Y.,Chen, H.,Zhou, Y.,Jiang, L.,Li, M.,Zhao, S.,Zhang, Y.
Alternative Z-genome biosynthesis pathway shows evolutionary progression from Archaea to phage.
Nat Microbiol, 8:1330-1338, 2023

PubMed Abstract: Many bacteriophages evade bacterial immune recognition by substituting adenine with 2,6-diaminopurine (Z) in their genomes. The Z-genome biosynthetic pathway involves PurZ that belongs to the PurA (adenylosuccinate synthetase) family and bears particular similarity to archaeal PurA. However, how the transition of PurA to PurZ occurred during evolution is not clear; recapturing this process may shed light on the origin of Z-containing phages. Here we describe the computer-guided identification and biochemical characterization of a naturally existing PurZ variant, PurZ0, which uses guanosine triphosphate as the phosphate donor rather than the ATP used by PurZ. The atomic resolution structure of PurZ0 reveals a guanine nucleotide binding pocket highly analogous to that of archaeal PurA. Phylogenetic analyses suggest PurZ0 as an intermediate during the evolution of archaeal PurA to phage PurZ. Maintaining the balance of different purines necessitates further evolvement of guanosine triphosphate-using PurZ0 to ATP-using PurZ in adaptation to Z-genome life.

PubMed: 37308591
DOI: 10.1038/s41564-023-01410-1

実験手法

X-RAY DIFFRACTION (1.703 Å)