7VEX

Crystal Structure of GTP-bound Irgb6

7VEX の概要

• エントリーDOI: 10.2210/pdb7vex/pdb
• 分子名称: T-cell-specific guanine nucleotide triphosphate-binding protein 2, GUANOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: irgb6, irgs, immunity-related gtpase, (ifn)-inducible gtpase, p47 gtpase, tgtp, dynamin superfamily, immune system, hydrolase
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47853.90

構造登録者

Saijo-Hamano, Y.,Sakai, N.,Nitta, R. (登録日: 2021-09-10, 公開日: 2021-11-03, 最終更新日: 2023-11-29)

主引用文献

Saijo-Hamano, Y.,Sherif, A.A.,Pradipta, A.,Sasai, M.,Sakai, N.,Sakihama, Y.,Yamamoto, M.,Standley, D.M.,Nitta, R.
Structural basis of membrane recognition of Toxoplasma gondii vacuole by Irgb6.
Life Sci Alliance, 5:-, 2022

PubMed Abstract: The p47 immunity-related GTPase (IRG) Irgb6 plays a pioneering role in host defense against infection. Irgb6 is recruited to the parasitophorous vacuole membrane (PVM) formed by and disrupts it. Despite the importance of this process, the molecular mechanisms accounting for PVM recognition by Irgb6 remain elusive because of lack of structural information on Irgb6. Here we report the crystal structures of mouse Irgb6 in the GTP-bound and nucleotide-free forms. Irgb6 exhibits a similar overall architecture to other IRGs in which GTP binding induces conformational changes in both the dimerization interface and the membrane-binding interface. The membrane-binding interface of Irgb6 assumes a unique conformation, composed of N- and C-terminal helical regions forming a phospholipid binding site. In silico docking of phospholipids further revealed membrane-binding residues that were validated through mutagenesis and cell-based assays. Collectively, these data demonstrate a novel structural basis for Irgb6 to recognize PVM in a manner distinct from other IRGs.

PubMed: 34753804
DOI: 10.26508/lsa.202101149

実験手法

X-RAY DIFFRACTION (1.51 Å)