7VEE
The ligand-free structure of GfsA KSQ-AT didomain
Summary for 7VEE
| Entry DOI | 10.2210/pdb7vee/pdb |
| Descriptor | Polyketide synthase, GLYCEROL (3 entities in total) |
| Functional Keywords | decarboxylase, thiolase fold, polyketide biosynthesis, biosynthetic protein, transferase |
| Biological source | Streptomyces graminofaciens |
| Total number of polymer chains | 1 |
| Total formula weight | 95624.70 |
| Authors | Chisuga, T.,Miyanaga, A.,Nagai, A.,Kudo, F.,Eguchi, T. (deposition date: 2021-09-08, release date: 2022-01-12, Last modification date: 2023-11-29) |
| Primary citation | Chisuga, T.,Nagai, A.,Miyanaga, A.,Goto, E.,Kishikawa, K.,Kudo, F.,Eguchi, T. Structural Insight into the Reaction Mechanism of Ketosynthase-Like Decarboxylase in a Loading Module of Modular Polyketide Synthases. Acs Chem.Biol., 17:198-206, 2022 Cited by PubMed Abstract: Ketosynthase-like decarboxylase (KS) domains are widely distributed in the loading modules of modular polyketide synthases (PKSs) and are proposed to catalyze the decarboxylation of a malonyl or methylmalonyl unit for the construction of the PKS starter unit. KS domains have high sequence similarity to ketosynthase (KS) domains, which catalyze transacylation and decarboxylative condensation in polyketide and fatty acid biosynthesis, except that the catalytic Cys residue of KS domains is replaced by Gln in KS domains. Here, we present biochemical analyses of GfsA KS and CmiP4 KS, which are involved in the biosynthesis of FD-891 and cremimycin, respectively. analysis showed that these KS domains catalyze the decarboxylation of malonyl and methylmalonyl units. Furthermore, we determined the crystal structure of GfsA KS in complex with a malonyl thioester substrate analogue, which enabled identification of key amino acid residues involved in the decarboxylation reaction. The importance of these residues was confirmed by mutational analysis. On the basis of these findings, we propose a mechanism of the decarboxylation reaction catalyzed by GfsA KS. GfsA KS initiates decarboxylation by fixing the substrate in a suitable conformation for decarboxylation. The formation of enolate upon decarboxylation is assisted by two conserved threonine residues. Comparison of the structure of GfsA KS with those of KS domains suggests that the Gln residue in the active site of the KS domain mimics the acylated Cys residue in the active site of KS domains. PubMed: 34985877DOI: 10.1021/acschembio.1c00856 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.55 Å) |
Structure validation
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