7VBM

The mouse nucleosome structure containing H3mm18 aided by PL2-6 scFv

7VBM の概要

• エントリーDOI: 10.2210/pdb7vbm/pdb
• 関連するPDBエントリー: 7DBH
• EMDBエントリー: 30631 31882
• 分子名称: Histone H3mm18, Histone H4, Histone H2A type 1-B, ... (6 entities in total)
• 機能のキーワード: complex, chromatin, nucleosome, dna binding protein, dna binding protein-dna complex
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 201077.90

構造登録者

Hirai, S.,Takizawa, Y.,Kujirai, T.,Kurumizaka, H. (登録日: 2021-08-31, 公開日: 2022-01-19, 最終更新日: 2024-06-19)

主引用文献

Hirai, S.,Tomimatsu, K.,Miyawaki-Kuwakado, A.,Takizawa, Y.,Komatsu, T.,Tachibana, T.,Fukushima, Y.,Takeda, Y.,Negishi, L.,Kujirai, T.,Koyama, M.,Ohkawa, Y.,Kurumizaka, H.
Unusual nucleosome formation and transcriptome influence by the histone H3mm18 variant.
Nucleic Acids Res., 50:72-91, 2022

PubMed Abstract: Histone H3mm18 is a non-allelic H3 variant expressed in skeletal muscle and brain in mice. However, its function has remained enigmatic. We found that H3mm18 is incorporated into chromatin in cells with low efficiency, as compared to H3.3. We determined the structures of the nucleosome core particle (NCP) containing H3mm18 by cryo-electron microscopy, which revealed that the entry/exit DNA regions are drastically disordered in the H3mm18 NCP. Consistently, the H3mm18 NCP is substantially unstable in vitro. The forced expression of H3mm18 in mouse myoblast C2C12 cells markedly suppressed muscle differentiation. A transcriptome analysis revealed that the forced expression of H3mm18 affected the expression of multiple genes, and suppressed a group of genes involved in muscle development. These results suggest a novel gene expression regulation system in which the chromatin landscape is altered by the formation of unusual nucleosomes with a histone variant, H3mm18, and provide important insight into understanding transcription regulation by chromatin.

PubMed: 34929737
DOI: 10.1093/nar/gkab1137

実験手法

ELECTRON MICROSCOPY (3.4 Å)