7VBB

Structure of the post state human RNA Polymerase I Elongation Complex

This is a non-PDB format compatible entry.

Summary for 7VBB

• Entry DOI: 10.2210/pdb7vbb/pdb
• EMDB information: 31877
• Descriptor: DNA-directed RNA polymerase I subunit RPA1, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA34, ... (18 entities in total)
• Functional Keywords: rna polymerase i, transcription, post state, transcription-dna-rna complex, transcription/dna/rna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 16
• Total formula weight: 617396.32

Authors

Zhao, D.,Liu, W.,Chen, K.,Yang, H.,Xu, Y. (deposition date: 2021-08-31, release date: 2022-03-02, Last modification date: 2024-06-19)

Primary citation

Zhao, D.,Liu, W.,Chen, K.,Wu, Z.,Yang, H.,Xu, Y.
Structure of the human RNA polymerase I elongation complex.
Cell Discov, 7:97-97, 2021

PubMed Abstract: Eukaryotic RNA polymerase I (Pol I) transcribes ribosomal DNA and generates RNA for ribosome synthesis. Pol I accounts for the majority of cellular transcription activity and dysregulation of Pol I transcription leads to cancers and ribosomopathies. Despite extensive structural studies of yeast Pol I, structure of human Pol I remains unsolved. Here we determined the structures of the human Pol I in the pre-translocation, post-translocation, and backtracked states at near-atomic resolution. The single-subunit peripheral stalk lacks contacts with the DNA-binding clamp and is more flexible than the two-subunit stalk in yeast Pol I. Compared to yeast Pol I, human Pol I possesses a more closed clamp, which makes more contacts with DNA. The Pol I structure in the post-cleavage backtracked state shows that the C-terminal zinc ribbon of RPA12 inserts into an open funnel and facilitates "dinucleotide cleavage" on mismatched DNA-RNA hybrid. Critical disease-associated mutations are mapped on Pol I regions that are involved in catalysis and complex organization. In summary, the structures provide new sights into human Pol I complex organization and efficient proofreading.

PubMed: 34671025
DOI: 10.1038/s41421-021-00335-5

Experimental method

ELECTRON MICROSCOPY (2.81 Å)