7V9F

Selenomethionine mutant (L740Sem) of BEN4 domain of protein Bend3 with DNA

7V9F の概要

• エントリーDOI: 10.2210/pdb7v9f/pdb
• 分子名称: BEN domain-containing protein 3, DNA (5'-D(*TP*GP*GP*CP*CP*CP*CP*AP*CP*GP*CP*GP*GP*TP*GP*C)-3'), DNA (5'-D(*GP*CP*AP*CP*CP*GP*CP*GP*TP*GP*GP*GP*GP*CP*CP*A)-3'), ... (5 entities in total)
• 機能のキーワード: dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 23834.19

構造登録者

Zhang, J.,Zhang, Y.,You, Q.,Huang, C.,Zhang, T.,Wang, M.,Zhang, T.,Yang, X.,Xiong, J.,Li, Y.,Liu, C.P.,Zhang, Z.,Xu, R.M.,Zhu, B. (登録日: 2021-08-25, 公開日: 2022-02-16, 最終更新日: 2024-10-16)

主引用文献

Zhang, J.,Zhang, Y.,You, Q.,Huang, C.,Zhang, T.,Wang, M.,Zhang, T.,Yang, X.,Xiong, J.,Li, Y.,Liu, C.P.,Zhang, Z.,Xu, R.M.,Zhu, B.
Highly enriched BEND3 prevents the premature activation of bivalent genes during differentiation.
Science, 375:1053-1058, 2022

PubMed Abstract: Bivalent genes are ready for activation upon the arrival of developmental cues. Here, we report that BEND3 is a CpG island (CGI)-binding protein that is enriched at regulatory elements. The cocrystal structure of BEND3 in complex with its target DNA reveals the structural basis for its DNA methylation-sensitive binding property. Mouse embryos ablated of died at the pregastrulation stage. null embryonic stem cells (ESCs) exhibited severe defects in differentiation, during which hundreds of CGI-containing bivalent genes were prematurely activated. BEND3 is required for the stable association of polycomb repressive complex 2 (PRC2) at bivalent genes that are highly occupied by BEND3, which suggests a reining function of BEND3 in maintaining high levels of H3K27me3 at these bivalent genes in ESCs to prevent their premature activation in the forthcoming developmental stage.

PubMed: 35143257
DOI: 10.1126/science.abm0730

実験手法

X-RAY DIFFRACTION (2.5 Å)