7V9E

Crystal structure of a methyl transferase ribozyme

7V9E の概要

• エントリーDOI: 10.2210/pdb7v9e/pdb
• 分子名称: RNA (68-MER), GUANINE, BARIUM ION, ... (5 entities in total)
• 機能のキーワード: methyl transferase, ribozyme, riboswitch, rna
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23317.18

構造登録者

Deng, J.,Lilley, D.M.J.,Huang, L. (登録日: 2021-08-25, 公開日: 2022-03-23, 最終更新日: 2024-05-29)

主引用文献

Deng, J.,Wilson, T.J.,Wang, J.,Peng, X.,Li, M.,Lin, X.,Liao, W.,Lilley, D.M.J.,Huang, L.
Structure and mechanism of a methyltransferase ribozyme.
Nat.Chem.Biol., 18:556-564, 2022

PubMed Abstract: Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but in vitro selection has generated species that catalyze a broader range of chemistry; yet, there have been few structural and mechanistic studies of selected ribozymes. A ribozyme has recently been selected that can catalyze a site-specific methyl transfer reaction. We have solved the crystal structure of this ribozyme at a resolution of 2.3 Å, showing how the RNA folds to generate a very specific binding site for the methyl donor substrate. The structure immediately suggests a catalytic mechanism involving a combination of proximity and orientation and nucleobase-mediated general acid catalysis. The mechanism is supported by the pH dependence of the rate of catalysis. A selected methyltransferase ribozyme can thus use a relatively sophisticated catalytic mechanism, broadening the range of known RNA-catalyzed chemistry.

PubMed: 35301479
DOI: 10.1038/s41589-022-00982-z

実験手法

X-RAY DIFFRACTION (2.3 Å)