7UZT

Crystal structure of the human astrovirus MLB1 capsid protein spike domain at 1.86-A resolution

7UZT の概要

• エントリーDOI: 10.2210/pdb7uzt/pdb
• 分子名称: Capsid polyprotein VP90 (2 entities in total)
• 機能のキーワード: capsid protein, spike domain, projection domain, viral protein
• 由来する生物種: Astrovirus MLB1
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 88924.57

構造登録者

Delgado-Cunningham, K.,DuBois, R. (登録日: 2022-05-09, 公開日: 2022-11-09, 最終更新日: 2024-05-22)

主引用文献

Delgado-Cunningham, K.,Lopez, T.,Khatib, F.,Arias, C.F.,DuBois, R.M.
Structure of the divergent human astrovirus MLB capsid spike.
Structure, 30:1573-1581.e3, 2022

PubMed Abstract: Despite their worldwide prevalence and association with human disease, the molecular bases of human astrovirus (HAstV) infection and evolution remain poorly characterized. Here, we report the structure of the capsid protein spike of the divergent HAstV MLB clade (HAstV MLB). While the structure shares a similar folding topology with that of classical-clade HAstV spikes, it is otherwise strikingly different. We find no evidence of a conserved receptor-binding site between the MLB and classical HAstV spikes, suggesting that MLB and classical HAstVs utilize different receptors for host-cell attachment. We provide evidence for this hypothesis using a novel HAstV infection competition assay. Comparisons of the HAstV MLB spike structure with structures predicted from its sequence reveal poor matches, but template-based predictions were surprisingly accurate relative to machine-learning-based predictions. Our data provide a foundation for understanding the mechanisms of infection by diverse HAstVs and can support structure determination in similarly unstudied systems.

PubMed: 36417907
DOI: 10.1016/j.str.2022.10.010

実験手法

X-RAY DIFFRACTION (1.86 Å)