7UYI

Crystal structure of the computationally optimized broadly reactive H1 influenza hemagglutinin P1

7UYI の概要

• エントリーDOI: 10.2210/pdb7uyi/pdb
• 分子名称: COBRA P1 HA, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: hemagglutinin, virus entry, antigenic, viral protein
• 由来する生物種: Influenza A virus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 356482.86

構造登録者

Dzimianski, J.V.,DuBois, R.M. (登録日: 2022-05-06, 公開日: 2023-04-19, 最終更新日: 2024-10-09)

主引用文献

Dzimianski, J.V.,Han, J.,Sautto, G.A.,O'Rourke, S.M.,Cruz, J.M.,Pierce, S.R.,Ecker, J.W.,Carlock, M.A.,Nagashima, K.A.,Mousa, J.J.,Ross, T.M.,Ward, A.B.,DuBois, R.M.
Structural insights into the broad protection against H1 influenza viruses by a computationally optimized hemagglutinin vaccine.
Commun Biol, 6:454-454, 2023

PubMed Abstract: Influenza virus poses an ongoing human health threat with pandemic potential. Due to mutations in circulating strains, formulating effective vaccines remains a challenge. The use of computationally optimized broadly reactive antigen (COBRA) hemagglutinin (HA) proteins is a promising vaccine strategy to protect against a wide range of current and future influenza viruses. Though effective in preclinical studies, the mechanistic basis driving the broad reactivity of COBRA proteins remains to be elucidated. Here, we report the crystal structure of the COBRA HA termed P1 and identify antigenic and glycosylation properties that contribute to its immunogenicity. We further report the cryo-EM structure of the P1-elicited broadly neutralizing antibody 1F8 bound to COBRA P1, revealing 1F8 to recognize an atypical receptor binding site epitope via an unexpected mode of binding.

PubMed: 37185989
DOI: 10.1038/s42003-023-04793-3

実験手法

X-RAY DIFFRACTION (3 Å)