7UXB

Human triosephosphate isomerase mutant G122R

7UXB の概要

• エントリーDOI: 10.2210/pdb7uxb/pdb
• 分子名称: Triosephosphate isomerase, ISOPROPYL ALCOHOL, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: enzyme, glycolysis, isomerase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54031.68

構造登録者

Romero, J.M. (登録日: 2022-05-05, 公開日: 2023-05-24, 最終更新日: 2024-11-20)

主引用文献

Romero, J.M.
Structural analysis of the TPI-Manchester, a thermolabile variant of human triosephosphate isomerase.
Arch.Biochem.Biophys., 761:110156-110156, 2024

PubMed Abstract: Human triosephosphate isomerase G122R, also known as TPI-Manchester, is a thermolabile variant detected in a screening of more than 3400 individuals from a population in Ann Arbor, Michigan. Here, the crystallographic structure of G122R was solved to determine the molecular basis of its thermal stability. Structural analysis revealed an increase in the flexibility of residues at the dimer interface, even though R122 is about 20 Å away, suggesting that long-range electrostatic interactions may play a key role in the mutation effect.

PubMed: 39299479
DOI: 10.1016/j.abb.2024.110156

実験手法

X-RAY DIFFRACTION (2 Å)