7UWS

Atomic model of the partial VSV nucleocapsid

7UWS の概要

• エントリーDOI: 10.2210/pdb7uws/pdb
• EMDBエントリー: 26841
• 分子名称: Nucleoprotein, RNA, Matrix protein (3 entities in total)
• 機能のキーワード: vesicular stomatitis virus, nucleocapsid, matrix protein m, nucleocapsid protein n, viral protein-rna complex, viral protein/rna
• 由来する生物種: Vesicular stomatitis virus; 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 763589.78

構造登録者

Zhou, K.,Si, Z.,Ge, P.,Tsao, J.,Luo, M.,Zhou, Z.H. (登録日: 2022-05-03, 公開日: 2022-09-28, 最終更新日: 2024-06-12)

主引用文献

Zhou, K.,Si, Z.,Ge, P.,Tsao, J.,Luo, M.,Zhou, Z.H.
Atomic model of vesicular stomatitis virus and mechanism of assembly.
Nat Commun, 13:5980-5980, 2022

PubMed Abstract: Like other negative-strand RNA viruses (NSVs) such as influenza and rabies, vesicular stomatitis virus (VSV) has a three-layered organization: a layer of matrix protein (M) resides between the glycoprotein (G)-studded membrane envelope and the nucleocapsid, which is composed of the nucleocapsid protein (N) and the encapsidated genomic RNA. Lack of in situ atomic structures of these viral components has limited mechanistic understanding of assembling the bullet-shaped virion. Here, by cryoEM and sub-particle reconstruction, we have determined the in situ structures of M and N inside VSV at 3.47 Å resolution. In the virion, N and M sites have a stoichiometry of 1:2. The in situ structures of both N and M differ from their crystal structures in their N-terminal segments and oligomerization loops. N-RNA, N-N, and N-M-M interactions govern the formation of the capsid. A double layer of M contributes to packaging of the helical nucleocapsid: the inner M (IM) joins neighboring turns of the N helix, while the outer M (OM) contacts G and the membrane envelope. The pseudo-crystalline organization of G is further mapped by cryoET. The mechanism of VSV assembly is delineated by the network interactions of these viral components.

PubMed: 36216930
DOI: 10.1038/s41467-022-33664-4

実験手法

ELECTRON MICROSCOPY (3.47 Å)