7UW3

Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain

Summary for 7UW3

• Entry DOI: 10.2210/pdb7uw3/pdb
• Descriptor: Nucleoprotein (2 entities in total)
• Functional Keywords: covid-19, nucleoprotein, n-terminal domain, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus (SARS-CoV-2)
• Total number of polymer chains: 4
• Total formula weight: 59978.93

Authors

Calero, G. (deposition date: 2022-05-02, release date: 2023-05-10, Last modification date: 2023-10-25)

Primary citation

Sarkar, S.,Runge, B.,Russell, R.W.,Movellan, K.T.,Calero, D.,Zeinalilathori, S.,Quinn, C.M.,Lu, M.,Calero, G.,Gronenborn, A.M.,Polenova, T.
Atomic-Resolution Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain.
J.Am.Chem.Soc., 144:10543-10555, 2022

PubMed Abstract: The nucleocapsid (N) protein is one of the four structural proteins of the SARS-CoV-2 virus and plays a crucial role in viral genome organization and, hence, replication and pathogenicity. The N-terminal domain (N) binds to the genomic RNA and thus comprises a potential target for inhibitor and vaccine development. We determined the atomic-resolution structure of crystalline N by integrating solid-state magic angle spinning (MAS) NMR and X-ray diffraction. Our combined approach provides atomic details of protein packing interfaces as well as information about flexible regions as the N- and C-termini and the functionally important RNA binding, β-hairpin loop. In addition, ultrafast (100 kHz) MAS H-detected experiments permitted the assignment of side-chain proton chemical shifts not available by other means. The present structure offers guidance for designing therapeutic interventions against the SARS-CoV-2 infection.

PubMed: 35638584
DOI: 10.1021/jacs.2c03320

Experimental method

X-RAY DIFFRACTION (1.7 Å)