7US5

X-ray crystal structure of GDP-D-glycero-D-manno-heptose 4,6-Dehydratase from Campylobacter jejuni

7US5 の概要

• エントリーDOI: 10.2210/pdb7us5/pdb
• 分子名称: GDP-D-GLYCERO-D-MANNO-HEPTOSE 4,6-DEHYDRATASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: dehydratase, capsular polysaccharide, oxidoreductase
• 由来する生物種: Campylobacter jejuni
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 171232.16

構造登録者

Thoden, J.B.,Xiang, D.F.,Raushel, F.M.,Holden, H.M. (登録日: 2022-04-23, 公開日: 2022-07-06, 最終更新日: 2023-10-18)

主引用文献

Xiang, D.F.,Thoden, J.B.,Ghosh, M.K.,Holden, H.M.,Raushel, F.M.
Reaction Mechanism and Three-Dimensional Structure of GDP-d-glycero-alpha-d-manno-heptose 4,6-Dehydratase from Campylobacter jejuni.
Biochemistry, 61:1313-1322, 2022

PubMed Abstract: is a human pathogen and a leading cause of food poisoning in the United States and Europe. Surrounding the outside of the bacterium is a carbohydrate coat known as the capsular polysaccharide. Various strains of have different sequences of unusual sugars and an assortment of decorations. Many of the serotypes have heptoses with differing stereochemical arrangements at C2 through C6. One of the many common modifications is a 6-deoxy-heptose that is formed by dehydration of GDP-d-glycero-α-d-manno-heptose to GDP-6-deoxy-4-keto-d-lyxo-heptose via the action of the enzyme GDP-d-glycero-α-d-manno-heptose 4,6-dehydratase. Herein, we report the biochemical and structural characterization of this enzyme from 81-176 (serotype HS:23/36). The enzyme was purified to homogeneity, and its three-dimensional structure was determined to a resolution of 2.1 Å. Kinetic analyses suggest that the reaction mechanism proceeds through the formation of a 4-keto intermediate followed by the loss of water from C5/C6. Based on the three-dimensional structure, it is proposed that oxidation of C4 is assisted by proton transfer from the hydroxyl group to the phenolate of Tyr-159 and hydride transfer to the tightly bound NAD in the active site. Elimination of water at C5/C6 is most likely assisted by abstraction of the proton at C5 by Glu-136 and subsequent proton transfer to the hydroxyl at C6 via Ser-134 and Tyr-159. A bioinformatic analysis identified 19 additional 4,6-dehydratases from serotyped strains of that are 89-98% identical in the amino acid sequence, indicating that each of these strains should contain a 6-deoxy-heptose within their capsular polysaccharides.

PubMed: 35715226
DOI: 10.1021/acs.biochem.2c00244

実験手法

X-RAY DIFFRACTION (2.1 Å)