7UQA

Crystal structure of the small Ultra-Red Fluorescent Protein (smURFP)

7UQA の概要

• エントリーDOI: 10.2210/pdb7uqa/pdb
• 分子名称: small Ultra-Red Fluorescent Protein (smURFP), CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: smurfp, far-red, optoacoustic, phycobiliprotein, fluorescent protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 96599.98

構造登録者

Maiti, A.,Buffalo, C.Z.,Saurabh, S.,Montecinos-Franjola, F.,Hachey, J.S.,Conlon, W.J.,Tran, G.N.,Drobizhev, M.,Moerner, W.E.,Ghosh, P.,Matsuo, H.,Tsien, R.Y.,Lin, J.Y.,Rodriguez, E.A. (登録日: 2022-04-19, 公開日: 2023-07-19, 最終更新日: 2023-10-25)

主引用文献

Maiti, A.,Buffalo, C.Z.,Saurabh, S.,Montecinos-Franjola, F.,Hachey, J.S.,Conlon, W.J.,Tran, G.N.,Hassan, B.,Walters, K.J.,Drobizhev, M.,Moerner, W.E.,Ghosh, P.,Matsuo, H.,Tsien, R.Y.,Lin, J.Y.,Rodriguez, E.A.
Structural and photophysical characterization of the small ultra-red fluorescent protein.
Nat Commun, 14:4155-4155, 2023

PubMed Abstract: The small Ultra-Red Fluorescent Protein (smURFP) represents a new class of fluorescent protein with exceptional photostability and brightness derived from allophycocyanin in a previous directed evolution. Here, we report the smURFP crystal structure to better understand properties and enable further engineering of improved variants. We compare this structure to the structures of allophycocyanin and smURFP mutants to identify the structural origins of the molecular brightness. We then use a structure-guided approach to develop monomeric smURFP variants that fluoresce with phycocyanobilin but not biliverdin. Furthermore, we measure smURFP photophysical properties necessary for advanced imaging modalities, such as those relevant for two-photon, fluorescence lifetime, and single-molecule imaging. We observe that smURFP has the largest two-photon cross-section measured for a fluorescent protein, and that it produces more photons than organic dyes. Altogether, this study expands our understanding of the smURFP, which will inform future engineering toward optimal FPs compatible with whole organism studies.

PubMed: 37438348
DOI: 10.1038/s41467-023-39776-9

実験手法

X-RAY DIFFRACTION (2.802 Å)