7UNM

Human TMEM175 in an closed state

7UNM の概要

• エントリーDOI: 10.2210/pdb7unm/pdb
• EMDBエントリー: 26627
• 分子名称: Endosomal/lysosomal potassium channel TMEM175, POTASSIUM ION (3 entities in total)
• 機能のキーワード: potassium channel, membrane protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111529.93

構造登録者

Oh, S.,Hite, R.K. (登録日: 2022-04-11, 公開日: 2022-06-29, 最終更新日: 2024-02-14)

主引用文献

Oh, S.,Marinelli, F.,Zhou, W.,Lee, J.,Choi, H.J.,Kim, M.,Faraldo-Gomez, J.D.,Hite, R.K.
Differential ion dehydration energetics explains selectivity in the non-canonical lysosomal K + channel TMEM175.
Elife, 11:-, 2022

PubMed Abstract: Structures of the human lysosomal K channel transmembrane protein 175 (TMEM175) in open and closed states revealed a novel architecture lacking the canonical K selectivity filter motif present in previously known K channel structures. A hydrophobic constriction composed of four isoleucine residues was resolved in the pore and proposed to serve as the gate in the closed state, and to confer ion selectivity in the open state. Here, we achieve higher-resolution structures of the open and closed states and employ molecular dynamics simulations to analyze the conducting properties of the putative open state, demonstrating that it is permeable to K and, to a lesser degree, also Na. Both cations must dehydrate significantly to penetrate the narrow hydrophobic constriction, but ion flow is assisted by a favorable electrostatic field generated by the protein that spans the length of the pore. The balance of these opposing energetic factors explains why permeation is feasible, and why TMEM175 is selective for K over Na, despite the absence of the canonical selectivity filter. Accordingly, mutagenesis experiments reveal an exquisite sensitivity of the channel to perturbations that mitigate the constriction. Together, these data reveal a novel mechanism for selective permeation of ions by TMEM175 that is unlike that of other K channels.

PubMed: 35608336
DOI: 10.7554/eLife.75122

実験手法

ELECTRON MICROSCOPY (2.61 Å)