7U8A

Product of 14mer primer with activated G monomer diastereomer 2

7U8A の概要

• エントリーDOI: 10.2210/pdb7u8a/pdb
• 分子名称: RNA (5'-R(*(LKC)P*(LCC)P*(LCC)P*(LCG)P*AP*CP*UP*UP*AP*AP*GP*UP*CP*GP*(G46))-3'), 5'-O-[(R)-(2-amino-1H-imidazol-1-yl)(sulfanyl)phosphoryl]guanosine, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: rna duplex, phosphorothioate, non-enzymatic rna extension, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10598.44

構造登録者

Fang, Z.,Szostak, J.W. (登録日: 2022-03-08, 公開日: 2023-03-15, 最終更新日: 2024-04-17)

主引用文献

Fang, Z.,Pazienza, L.T.,Zhang, J.,Tam, C.P.,Szostak, J.W.
Catalytic Metal Ion-Substrate Coordination during Nonenzymatic RNA Primer Extension.
J.Am.Chem.Soc., 2024

PubMed Abstract: Nonenzymatic template-directed RNA copying requires catalysis by divalent metal ions. The primer extension reaction involves the attack of the primer 3'-hydroxyl on the adjacent phosphate of a 5'-5'-imidazolium-bridged dinucleotide substrate. However, the nature of the interaction of the catalytic metal ion with the reaction center remains unclear. To explore the coordination of the catalytic metal ion with the imidazolium-bridged dinucleotide substrate, we examined catalysis by oxophilic and thiophilic metal ions with both diastereomers of phosphorothioate-modified substrates. We show that Mg and Cd exhibit opposite preferences for the two phosphorothioate substrate diastereomers, indicating a stereospecific interaction of the divalent cation with one of the nonbridging phosphorus substituents. High-resolution X-ray crystal structures of the products of primer extension with phosphorothioate substrates reveal the absolute stereochemistry of this interaction and indicate that catalysis by Mg involves inner-sphere coordination with the nonbridging phosphate oxygen in the pro- position, while thiophilic cadmium ions interact with sulfur in the same position, as in one of the two phosphorothioate substrates. These results collectively suggest that during nonenzymatic RNA primer extension with a 5'-5'-imidazolium-bridged dinucleotide substrate the interaction of the catalytic Mg ion with the pro- oxygen of the reactive phosphate plays a crucial role in the metal-catalyzed S2(P) reaction.

PubMed: 38579124
DOI: 10.1021/jacs.4c00323

実験手法

X-RAY DIFFRACTION (2.1 Å)