7U5D

I-F3b Cascade-TniQ full R-loop complex

7U5D の概要

• エントリーDOI: 10.2210/pdb7u5d/pdb
• EMDBエントリー: 26348
• 分子名称: crRNA, Target strand DNA, Non-target strand DNA, ... (7 entities in total)
• 機能のキーワード: crispr-cas, transposon, cast, cascade, i-f, i-f3, dna binding protein, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• 由来する生物種: Aeromonas salmonicida; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 520484.51

構造登録者

Park, J.U.,Mehrotra, E.,Kellogg, E.H. (登録日: 2022-03-02, 公開日: 2023-06-21, 最終更新日: 2024-06-12)

主引用文献

Park, J.U.,Petassi, M.T.,Hsieh, S.C.,Mehrotra, E.,Schuler, G.,Budhathoki, J.,Truong, V.H.,Thyme, S.B.,Ke, A.,Kellogg, E.H.,Peters, J.E.
Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition.
Mol.Cell, 83:1827-1838.e6, 2023

PubMed Abstract: CRISPR-associated transposons (CASTs) are natural RNA-directed transposition systems. We demonstrate that transposon protein TniQ plays a central role in promoting R-loop formation by RNA-guided DNA-targeting modules. TniQ residues, proximal to CRISPR RNA (crRNA), are required for recognizing different crRNA categories, revealing an unappreciated role of TniQ to direct transposition into different classes of crRNA targets. To investigate adaptations allowing CAST elements to utilize attachment sites inaccessible to CRISPR-Cas surveillance complexes, we compared and contrasted PAM sequence requirements in both I-F3b CAST and I-F1 CRISPR-Cas systems. We identify specific amino acids that enable a wider range of PAM sequences to be accommodated in I-F3b CAST elements compared with I-F1 CRISPR-Cas, enabling CAST elements to access attachment sites as sequences drift and evade host surveillance. Together, this evidence points to the central role of TniQ in facilitating the acquisition of CRISPR effector complexes for RNA-guided DNA transposition.

PubMed: 37267904
DOI: 10.1016/j.molcel.2023.05.005

実験手法

ELECTRON MICROSCOPY (3.52 Å)