7U21

Human Class I MHC HLA-A2 in complex with AVGSYVYSV peptide

7U21 の概要

• エントリーDOI: 10.2210/pdb7u21/pdb
• 分子名称: MHC class I antigen, A-2 alpha chain, Beta-2-microglobulin, PGM5 peptide (465-473) (H5Y), ... (4 entities in total)
• 機能のキーワード: class i major histocompatibility complex, hla-a*02, pgm5, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89355.20

構造登録者

Weiss, L.I.,Baker, B.M. (登録日: 2022-02-22, 公開日: 2022-06-01, 最終更新日: 2024-10-23)

主引用文献

Keller, G.L.J.,Weiss, L.I.,Baker, B.M.
Physicochemical Heuristics for Identifying High Fidelity, Near-Native Structural Models of Peptide/MHC Complexes.
Front Immunol, 13:887759-887759, 2022

PubMed Abstract: There is long-standing interest in accurately modeling the structural features of peptides bound and presented by class I MHC proteins. This interest has grown with the advent of rapid genome sequencing and the prospect of personalized, peptide-based cancer vaccines, as well as the development of molecular and cellular therapeutics based on T cell receptor recognition of peptide-MHC. However, while the speed and accessibility of peptide-MHC modeling has improved substantially over the years, improvements in accuracy have been modest. Accuracy is crucial in peptide-MHC modeling, as T cell receptors are highly sensitive to peptide conformation and capturing fine details is therefore necessary for useful models. Studying nonameric peptides presented by the common class I MHC protein HLA-A*02:01, here we addressed a key question common to modern modeling efforts: from a set of models (or decoys) generated through conformational sampling, which is best? We found that the common strategy of decoy selection by lowest energy can lead to substantial errors in predicted structures. We therefore adopted a data-driven approach and trained functions capable of predicting near native decoys with exceptionally high accuracy. Although our implementation is limited to nonamer/HLA-A*02:01 complexes, our results serve as an important proof of concept from which improvements can be made and, given the significance of HLA-A*02:01 and its preference for nonameric peptides, should have immediate utility in select immunotherapeutic and other efforts for which structural information would be advantageous.

PubMed: 35547730
DOI: 10.3389/fimmu.2022.887759

実験手法

X-RAY DIFFRACTION (1.9 Å)