7TVP

Viral AMG chitosanase V-Csn, E157Q mutant, chitotriose complex

7TVP の概要

• エントリーDOI: 10.2210/pdb7tvp/pdb
• 関連するPDBエントリー: 7TVL 7TVM 7TVN 7TVO
• 分子名称: Viral chitosanase V-Csn E157Q mutant chitotriose complex, 2-amino-2-deoxy-beta-D-glucopyranose-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: glycosyl hydrolase, chitosanase, viral auxiliary metabolic gene, mutant, chitosan complex, hydrolase
• 由来する生物種: unclassified sequences
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24756.14

構造登録者

Smith, C.A.,Wu, R.,Buchko, G.W.,Cort, J.R.,Hofmockel, K.S.,Jansson, J.K. (登録日: 2022-02-05, 公開日: 2022-10-05, 最終更新日: 2023-10-18)

主引用文献

Wu, R.,Smith, C.A.,Buchko, G.W.,Blaby, I.K.,Paez-Espino, D.,Kyrpides, N.C.,Yoshikuni, Y.,McDermott, J.E.,Hofmockel, K.S.,Cort, J.R.,Jansson, J.K.
Structural characterization of a soil viral auxiliary metabolic gene product - a functional chitosanase.
Nat Commun, 13:5485-5485, 2022

PubMed Abstract: Metagenomics is unearthing the previously hidden world of soil viruses. Many soil viral sequences in metagenomes contain putative auxiliary metabolic genes (AMGs) that are not associated with viral replication. Here, we establish that AMGs on soil viruses actually produce functional, active proteins. We focus on AMGs that potentially encode chitosanase enzymes that metabolize chitin - a common carbon polymer. We express and functionally screen several chitosanase genes identified from environmental metagenomes. One expressed protein showing endo-chitosanase activity (V-Csn) is crystalized and structurally characterized at ultra-high resolution, thus representing the structure of a soil viral AMG product. This structure provides details about the active site, and together with structure models determined using AlphaFold, facilitates understanding of substrate specificity and enzyme mechanism. Our findings support the hypothesis that soil viruses contribute auxiliary functions to their hosts.

PubMed: 36123347
DOI: 10.1038/s41467-022-32993-8

実験手法

X-RAY DIFFRACTION (1.3 Å)