7TV2

X-ray crystal structure of HIV-2 CA protein CTD

Summary for 7TV2

• Entry DOI: 10.2210/pdb7tv2/pdb
• Descriptor: Capsid protein p24 (2 entities in total)
• Functional Keywords: viral protein, structural protein
• Biological source: Human immunodeficiency virus type 2 (ISOLATE ROD)
• Total number of polymer chains: 1
• Total formula weight: 9713.20

Authors

Shi, K.,Aihara, H. (deposition date: 2022-02-03, release date: 2023-05-17, Last modification date: 2023-10-25)

Primary citation

Talledge, N.,Yang, H.,Shi, K.,Coray, R.,Yu, G.,Arndt, W.G.,Meng, S.,Baxter, G.C.,Mendonca, L.M.,Castano-Diez, D.,Aihara, H.,Mansky, L.M.,Zhang, W.
HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation.
J.Mol.Biol., 435:168143-168143, 2023

PubMed Abstract: Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used the single particle cryo-electron microscopy method to determine the HIV-2 Gag lattice structure for immature virions. The reconstruction map at 5.5 Å resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag lattice structures. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. We also solved a 1.98 Å resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation.

PubMed: 37150290
DOI: 10.1016/j.jmb.2023.168143

Experimental method

X-RAY DIFFRACTION (1.98 Å)