7TNC

M13F/G116F Pseudomonas aeruginosa azurin

7TNC の概要

• エントリーDOI: 10.2210/pdb7tnc/pdb
• 分子名称: Azurin, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, COPPER (II) ION, ... (5 entities in total)
• 機能のキーワード: electron transfer protein, electron transport
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14352.59

構造登録者

Liu, Y.,Lu, Y. (登録日: 2022-01-20, 公開日: 2023-01-25, 最終更新日: 2024-11-13)

主引用文献

Liu, Y.,Marshall, N.M.,Yu, S.S.,Kim, W.,Gao, Y.G.,Robinson, H.,Nilges, M.J.,Zhang, Y.,New, S.Y.,Lu, Y.
Structural Basis for the Effects of Phenylalanine on Tuning the Reduction Potential of Type 1 Copper in Azurin.
Inorg.Chem., 62:11618-11625, 2023

PubMed Abstract: In order to investigate the effects of the secondary coordination sphere in fine-tuning redox potentials (°') of type 1 blue copper (T1Cu) in cupredoxins, we have introduced M13F, M44F, and G116F mutations both individually and in combination in the secondary coordination sphere of the T1Cu center of azurin (Az) from . These variants were found to differentially influence the °' of T1Cu, with M13F Az decreasing °', M44F Az increasing °', and G116F Az showing a negligible effect. In addition, combining the M13F and M44F mutations increases °' by 26 mV relative to WT-Az, which is very close to the combined effect of °' by each mutation. Furthermore, combining G116F with either M13F or M44F mutation resulted in negative and positive cooperative effects, respectively. Crystal structures of M13F/M44F-Az, M13F/G116F-Az, and M44F/G116F-Az combined with that of G116F-Az reveal these changes arise from steric effects and fine-tuning of hydrogen bond networks around the copper-binding His117 residue. The insights gained from this study would provide another step toward the development of redox-active proteins with tunable redox properties for many biological and biotechnological applications.

PubMed: 37424080
DOI: 10.1021/acs.inorgchem.3c01365

実験手法

X-RAY DIFFRACTION (1.47 Å)