7TN2

Composite model of a Chd1-nucleosome complex in the nucleotide-free state derived from 2.3A and 2.7A Cryo-EM maps

7TN2 の概要

• エントリーDOI: 10.2210/pdb7tn2/pdb
• EMDBエントリー: 25479 25480
• 分子名称: Histone H3, Histone H4, Histone H2A type 1, ... (7 entities in total)
• 機能のキーワード: chd1, chromatin remodeling, atpase, dbd, nucleosome, remodeling, transcription, dna binding protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 380981.33

構造登録者

Nodelman, I.M.,Bowman, G.D.,Armache, J.-P. (登録日: 2022-01-20, 公開日: 2022-03-02, 最終更新日: 2024-02-21)

主引用文献

Nodelman, I.M.,Das, S.,Faustino, A.M.,Fried, S.D.,Bowman, G.D.,Armache, J.P.
Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state.
Nat.Struct.Mol.Biol., 29:121-129, 2022

PubMed Abstract: Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. Here we report a complex of the Chd1 remodeler bound to a nucleosome in a nucleotide-free state, determined by cryo-EM to 2.3 Å resolution. The remodeler stimulates the nucleosome to absorb an additional nucleotide on each strand at two different locations: on the tracking strand within the ATPase binding site and on the guide strand one helical turn from the ATPase motor. Remarkably, the additional nucleotide on the tracking strand is associated with a local transformation toward an A-form geometry, explaining how sequential ratcheting of each DNA strand occurs. The structure also reveals a histone-binding motif, ChEx, which can block opposing remodelers on the nucleosome and may allow Chd1 to participate in histone reorganization during transcription.

PubMed: 35173352
DOI: 10.1038/s41594-021-00719-x

実験手法

ELECTRON MICROSCOPY (2.3 Å)