7TGY

Prototypic SARS-CoV-2 G614 spike (closed form)

7TGY の概要

• エントリーDOI: 10.2210/pdb7tgy/pdb
• EMDBエントリー: 25887 25888 25889
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: prototypic sars-cov-2 g614 spike, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 420100.57

構造登録者

Ye, G.,Liu, B.,Li, F. (登録日: 2022-01-09, 公開日: 2022-03-09, 最終更新日: 2024-11-13)

主引用文献

Ye, G.,Liu, B.,Li, F.
Cryo-EM structure of a SARS-CoV-2 omicron spike protein ectodomain.
Nat Commun, 13:1214-1214, 2022

PubMed Abstract: The omicron variant of SARS-CoV-2 has been spreading rapidly across the globe. The virus-surface spike protein plays a critical role in the cell entry and immune evasion of SARS-CoV-2. Here we determined the 3.0 Å cryo-EM structure of the omicron spike protein ectodomain. In contrast to the original strain of SARS-CoV-2 where the receptor-binding domain (RBD) of the spike protein takes a mixture of open ("standing up") and closed ("lying down") conformations, the omicron spike molecules are predominantly in the open conformation, with one upright RBD ready for receptor binding. The open conformation of the omicron spike is stabilized by enhanced inter-domain and inter-subunit packing, which involves new mutations in the omicron strain. Moreover, the omicron spike has undergone extensive mutations in RBD regions where known neutralizing antibodies target, allowing the omicron variant to escape immune surveillance aimed at the original viral strain. The stable open conformation of the omicron spike sheds light on the cell entry and immune evasion mechanisms of the omicron variant.

PubMed: 35241675
DOI: 10.1038/s41467-022-28882-9

実験手法

ELECTRON MICROSCOPY (3 Å)