7TCZ

Human cytomegalovirus protease mutant (C84A, C87A, C138A, C202A) in complex with inhibitor

7TCZ の概要

• エントリーDOI: 10.2210/pdb7tcz/pdb
• 分子名称: Assemblin, [1-(2-oxopropyl)-4-phenyl-1H-1,2,3-triazol-5-yl]methyl benzylcarbamate (3 entities in total)
• 機能のキーワード: protease, viral protein, hyrdrolase-hydrolase inhibitor complex, hyrdrolase/hydrolase inhibitor
• 由来する生物種: Human betaherpesvirus 5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29325.81

構造登録者

Hulce, K.R.,Bohn, M.,Ongpipattanakul, C.,Jaishankar, P.,Renslo, A.R.,Craik, C.S. (登録日: 2021-12-29, 公開日: 2022-01-12, 最終更新日: 2024-10-23)

主引用文献

Hulce, K.R.,Jaishankar, P.,Lee, G.M.,Bohn, M.F.,Connelly, E.J.,Wucherer, K.,Ongpipattanakul, C.,Volk, R.F.,Chuo, S.W.,Arkin, M.R.,Renslo, A.R.,Craik, C.S.
Inhibiting a dynamic viral protease by targeting a non-catalytic cysteine.
Cell Chem Biol, 29:785-798.e19, 2022

PubMed Abstract: Viruses are responsible for some of the most deadly human diseases, yet available vaccines and antivirals address only a fraction of the potential viral human pathogens. Here, we provide a methodology for managing human herpesvirus (HHV) infection by covalently inactivating the HHV maturational protease via a conserved, non-catalytic cysteine (C161). Using human cytomegalovirus protease (HCMV Pr) as a model, we screened a library of disulfides to identify molecules that tether to C161 and inhibit proteolysis, then elaborated hits into irreversible HCMV Pr inhibitors that exhibit broad-spectrum inhibition of other HHV Pr homologs. We further developed an optimized tool compound targeted toward HCMV Pr and used an integrative structural biology and biochemical approach to demonstrate inhibitor stabilization of HCMV Pr homodimerization, exploiting a conformational equilibrium to block proteolysis. Irreversible HCMV Pr inhibition disrupts HCMV infectivity in cells, providing proof of principle for targeting proteolysis via a non-catalytic cysteine to manage viral infection.

PubMed: 35364007
DOI: 10.1016/j.chembiol.2022.03.007

実験手法

X-RAY DIFFRACTION (2.67 Å)