7TB4

Cryo-EM structure of the spike of SARS-CoV-2 Omicron variant of concern

7TB4 の概要

• エントリーDOI: 10.2210/pdb7tb4/pdb
• EMDBエントリー: 25792
• 分子名称: Surface glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: sars-cov-2, spike, omicron, variant of concern, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 410176.33

構造登録者

Zhou, T.,Tsybovsky, T.,Kwong, P.D. (登録日: 2021-12-21, 公開日: 2022-01-12, 最終更新日: 2024-10-23)

主引用文献

Wang, L.,Zhou, T.,Zhang, Y.,Yang, E.S.,Schramm, C.A.,Shi, W.,Pegu, A.,Oloninyi, O.K.,Ransier, A.,Darko, S.,Narpala, S.R.,Hatcher, C.,Martinez, D.R.,Tsybovsky, Y.,Phung, E.,Abiona, O.M.,Cale, E.M.,Chang, L.A.,Corbett, K.S.,DiPiazza, A.T.,Gordon, I.J.,Leung, K.,Liu, T.,Mason, R.D.,Nazzari, A.,Novik, L.,Olia, A.S.,Doria-Rose, N.A.,Stephens, T.,Stringham, C.D.,Talana, C.A.,Teng, I.T.,Wagner, D.,Widge, A.T.,Zhang, B.,Roederer, M.,Ledgerwood, J.E.,Ruckwardt, T.J.,Gaudinski, M.R.,Baric, R.S.,Graham, B.S.,McDermott, A.B.,Douek, D.C.,Kwong, P.D.,Mascola, J.R.,Sullivan, N.J.,Misasi, J.
Antibodies with potent and broad neutralizing activity against antigenically diverse and highly transmissible SARS-CoV-2 variants.
Biorxiv, 2021

PubMed Abstract: The emergence of highly transmissible SARS-CoV-2 variants of concern (VOC) that are resistant to therapeutic antibodies highlights the need for continuing discovery of broadly reactive antibodies. We identify four receptor-binding domain targeting antibodies from three early-outbreak convalescent donors with potent neutralizing activity against 12 variants including the B.1.1.7 and B.1.351 VOCs. Two of them are ultrapotent, with sub-nanomolar neutralization titers (IC50 <0.0006 to 0.0102 μ g/mL; IC80 < 0.0006 to 0.0251 μ g/mL). We define the structural and functional determinants of binding for all four VOC-targeting antibodies, and show that combinations of two antibodies decrease the in vitro generation of escape mutants, suggesting potential means to mitigate resistance development. These results define the basis of therapeutic cocktails against VOCs and suggest that targeted boosting of existing immunity may increase vaccine breadth against VOCs.

PubMed: 33655252
DOI: 10.1101/2021.02.25.432969

実験手法

ELECTRON MICROSCOPY (3.29 Å)