7T3Q

IP3 and ATP bound type 3 IP3 receptor in the pre-active B state

これはPDB形式変換不可エントリーです。

7T3Q の概要

• エントリーDOI: 10.2210/pdb7t3q/pdb
• EMDBエントリー: 25668
• 分子名称: Inositol 1,4,5-trisphosphate receptor type 3, ZINC ION, D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ip3 receptor, calcium signaling, metal transport
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 1201274.24

構造登録者

Schmitz, E.A.,Takahashi, H.,Karakas, E. (登録日: 2021-12-08, 公開日: 2022-03-23, 最終更新日: 2024-10-30)

主引用文献

Schmitz, E.A.,Takahashi, H.,Karakas, E.
Structural basis for activation and gating of IP 3 receptors.
Nat Commun, 13:1408-1408, 2022

PubMed Abstract: A pivotal component of the calcium (Ca) signaling toolbox in cells is the inositol 1,4,5-triphosphate (IP) receptor (IPR), which mediates Ca release from the endoplasmic reticulum (ER), controlling cytoplasmic and organellar Ca concentrations. IPRs are co-activated by IP and Ca, inhibited by Ca at high concentrations, and potentiated by ATP. However, the underlying molecular mechanisms are unclear. Here we report cryo-electron microscopy (cryo-EM) structures of human type-3 IPR obtained from a single dataset in multiple gating conformations: IP-ATP bound pre-active states with closed channels, IP-ATP-Ca bound active state with an open channel, and IP-ATP-Ca bound inactive state with a closed channel. The structures demonstrate how IP-induced conformational changes prime the receptor for activation by Ca, how Ca binding leads to channel opening, and how ATP modulates the activity, providing insights into the long-sought questions regarding the molecular mechanism underpinning receptor activation and gating.

PubMed: 35301323
DOI: 10.1038/s41467-022-29073-2

実験手法

ELECTRON MICROSCOPY (3.3 Å)