7T0F

Crystal structure of S25-39 Fab in complex with 4-MeO-KdoOAll

7T0F の概要

• エントリーDOI: 10.2210/pdb7t0f/pdb
• 関連するPDBエントリー: 7T0G 7T0H 7T0I 7T0J 7T0K
• 分子名称: S25-39 Fab heavy chain, S25-39 Fab light chain, 3-deoxy-4-O-methyl-alpha-D-manno-oct-2-ulopyranosonic acid, ... (4 entities in total)
• 機能のキーワード: antibody, fab, carbohydrate, induced fit, conformational selection, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48544.08

構造登録者

Legg, M.S.G.,Blackler, R.J.,Evans, S.V. (登録日: 2021-11-29, 公開日: 2022-04-20, 最終更新日: 2024-11-20)

主引用文献

Blackler, R.J.,Muller-Loennies, S.,Pokorny-Lehrer, B.,Legg, M.S.G.,Brade, L.,Brade, H.,Kosma, P.,Evans, S.V.
Antigen binding by conformational selection in near-germline antibodies.
J.Biol.Chem., 298:101901-101901, 2022

PubMed Abstract: Conformational flexibility in antibody-combining sites has been hypothesized to facilitate polyspecificity toward multiple unique epitopes and enable the limited germline repertoire to match an overwhelming diversity of potential antigens; however, elucidating the mechanisms of antigen recognition by flexible antibodies has been understandably challenging. Here, multiple liganded and unliganded crystal structures of the near-germline anticarbohydrate antibodies S25-2 and S25-39 are reported, which reveal an unprecedented diversity of complementarity-determining region H3 conformations in apparent equilibrium. These structures demonstrate that at least some germline or near-germline antibodies are flexible entities sensitive to their chemical environments, with conformational selection available as an evolved mechanism that preserves the inherited ability to recognize common pathogens while remaining adaptable to new threats.

PubMed: 35395245
DOI: 10.1016/j.jbc.2022.101901

実験手法

X-RAY DIFFRACTION (2 Å)