7SZT

Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters in low pH (protonated state)

7SZT の概要

• エントリーDOI: 10.2210/pdb7szt/pdb
• 分子名称: Multidrug resistance protein, SMR family, L10 monobody, Dodecyldimethylphosphine oxide (3 entities in total)
• 機能のキーワード: small multidrug resistance transporters, membrane protein, transport protein
• 由来する生物種: Clostridiales bacterium oral taxon 876; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 85587.93

構造登録者

Kermani, A.A.,Stockbridge, R.B.,Burata, O.E. (登録日: 2021-11-29, 公開日: 2022-03-02, 最終更新日: 2023-10-18)

主引用文献

Kermani, A.A.,Burata, O.E.,Koff, B.B.,Koide, A.,Koide, S.,Stockbridge, R.B.
Crystal structures of bacterial small multidrug resistance transporter EmrE in complex with structurally diverse substrates.
Elife, 11:-, 2022

PubMed Abstract: Proteins from the bacterial small multidrug resistance (SMR) family are proton-coupled exporters of diverse antiseptics and antimicrobials, including polyaromatic cations and quaternary ammonium compounds. The transport mechanism of the transporter, EmrE, has been studied extensively, but a lack of high-resolution structural information has impeded a structural description of its molecular mechanism. Here, we apply a novel approach, multipurpose crystallization chaperones, to solve several structures of EmrE, including a 2.9 Å structure at low pH without substrate. We report five additional structures in complex with structurally diverse transported substrates, including quaternary phosphonium, quaternary ammonium, and planar polyaromatic compounds. These structures show that binding site tryptophan and glutamate residues adopt different rotamers to conform to disparate structures without requiring major rearrangements of the backbone structure. Structural and functional comparison to Gdx-Clo, an SMR protein that transports a much narrower spectrum of substrates, suggests that in EmrE, a relatively sparse hydrogen bond network among binding site residues permits increased sidechain flexibility.

PubMed: 35254261
DOI: 10.7554/eLife.76766

実験手法

X-RAY DIFFRACTION (2.32 Å)