7SQU

Goslar chimallin C4 tetramer localized reconstruction

7SQU の概要

• エントリーDOI: 10.2210/pdb7squ/pdb
• EMDBエントリー: 25183 25220 25221 25222 25223 25229 25262 25358 25359 25360 25390 25391 25392 25393 25394 25395 25396
• 分子名称: Chimallin (1 entity in total)
• 機能のキーワード: phage, viral protein, structural protein
• 由来する生物種: Escherichia phage vB_EcoM_Goslar
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 838673.34

構造登録者

Laughlin, T.G.,Deep, A.,Prichard, A.M.,Seitz, C.,Gu, Y.,Enustun, E.,Suslov, S.,Khanna, K.,Birkholz, E.A.,Amaro, R.E.,Pogliano, J.,Corbett, K.D.,Villa, E. (登録日: 2021-11-06, 公開日: 2022-07-27, 最終更新日: 2024-06-05)

主引用文献

Laughlin, T.G.,Deep, A.,Prichard, A.M.,Seitz, C.,Gu, Y.,Enustun, E.,Suslov, S.,Khanna, K.,Birkholz, E.A.,Armbruster, E.,McCammon, J.A.,Amaro, R.E.,Pogliano, J.,Corbett, K.D.,Villa, E.
Architecture and self-assembly of the jumbo bacteriophage nuclear shell.
Nature, 608:429-435, 2022

PubMed Abstract: Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors. However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.

PubMed: 35922510
DOI: 10.1038/s41586-022-05013-4

実験手法

ELECTRON MICROSCOPY (2.6 Å)