7SPZ

Nucleotide-free Get3 in two open forms

7SPZ の概要

• エントリーDOI: 10.2210/pdb7spz/pdb
• 分子名称: ATPase ASNA1 homolog, ZINC ION (2 entities in total)
• 機能のキーワード: tail-anchored membrane protein targeting deviant walker a atpase targeting factor, chaperone
• 由来する生物種: Giardia lamblia ATCC 50803
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78524.01

構造登録者

Fry, M.Y.,Maggiolo, A.O.,Clemons Jr., W.M. (登録日: 2021-11-04, 公開日: 2022-07-20, 最終更新日: 2023-10-18)

主引用文献

Fry, M.Y.,Najdrova, V.,Maggiolo, A.O.,Saladi, S.M.,Dolezal, P.,Clemons Jr., W.M.
Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.
Nat.Struct.Mol.Biol., 29:820-830, 2022

PubMed Abstract: Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.

PubMed: 35851188
DOI: 10.1038/s41594-022-00798-4

実験手法

X-RAY DIFFRACTION (3 Å)