7SPD

Crystal Structure of The Tetramerization Domain (29-147) From Human Voltage-gated Potassium Channel Kv2.1 in C 2 2 21 Space Group

7SPD の概要

• エントリーDOI: 10.2210/pdb7spd/pdb
• 分子名称: Potassium voltage-gated channel subfamily B member 1, DI(HYDROXYETHYL)ETHER, ZINC ION, ... (4 entities in total)
• 機能のキーワード: pentamer, potassium voltage-gated channel, t1 domain, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 74281.64

構造登録者

Xu, Z.,Schnicker, N.,Baker, S. (登録日: 2021-11-02, 公開日: 2022-06-08, 最終更新日: 2023-10-18)

主引用文献

Xu, Z.,Khan, S.,Schnicker, N.J.,Baker, S.
Pentameric assembly of the Kv2.1 tetramerization domain.
Acta Crystallogr D Struct Biol, 78:792-802, 2022

PubMed Abstract: The Kv family of voltage-gated potassium channels regulate neuronal excitability. The biophysical characteristics of Kv channels can be matched to the needs of different neurons by forming homotetrameric or heterotetrameric channels within one of four subfamilies. The cytoplasmic tetramerization (T1) domain plays a major role in dictating the compatibility of different Kv subunits. The only Kv subfamily lacking a representative structure of the T1 domain is the Kv2 family. Here, X-ray crystallography was used to solve the structure of the human Kv2.1 T1 domain. The structure is similar to those of other T1 domains, but surprisingly formed a pentamer instead of a tetramer. In solution the Kv2.1 T1 domain also formed a pentamer, as determined by inline SEC-MALS-SAXS and negative-stain electron microscopy. The Kv2.1 T1-T1 interface involves electrostatic interactions, including a salt bridge formed by the negative charges in a previously described CDD motif, and inter-subunit coordination of zinc. It is shown that zinc binding is important for stability. In conclusion, the Kv2.1 T1 domain behaves differently from the other Kv T1 domains, which may reflect the versatility of Kv2.1, which can assemble with the regulatory KvS subunits and scaffold ER-plasma membrane contacts.

PubMed: 35647925
DOI: 10.1107/S205979832200568X

実験手法

X-RAY DIFFRACTION (2.7 Å)