7SKA

Sub-tomogram averaged structure of HIV-1 Envelope protein in native membrane

Summary for 7SKA

• Entry DOI: 10.2210/pdb7ska/pdb
• EMDB information: 25178 25186 25809
• Descriptor: Envelope glycoprotein gp120, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (13 entities in total)
• Functional Keywords: hiv-1, envelope protein, glycoprotein, env, membrane bound, viral protein
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 6
• Total formula weight: 244801.51

Authors

Mangala Prasad, V.,Lee, K.K. (deposition date: 2021-10-20, release date: 2022-03-09, Last modification date: 2024-10-23)

Primary citation

Mangala Prasad, V.,Leaman, D.P.,Lovendahl, K.N.,Croft, J.T.,Benhaim, M.A.,Hodge, E.A.,Zwick, M.B.,Lee, K.K.
Cryo-ET of Env on intact HIV virions reveals structural variation and positioning on the Gag lattice.
Cell, 185:641-653.e17, 2022

PubMed Abstract: HIV-1 Env mediates viral entry into host cells and is the sole target for neutralizing antibodies. However, Env structure and organization in its native virion context has eluded detailed characterization. Here, we used cryo-electron tomography to analyze Env in mature and immature HIV-1 particles. Immature particles showed distinct Env positioning relative to the underlying Gag lattice, providing insights into long-standing questions about Env incorporation. A 9.1-Å sub-tomogram-averaged reconstruction of virion-bound Env in conjunction with structural mass spectrometry revealed unexpected features, including a variable central core of the gp41 subunit, heterogeneous glycosylation between protomers, and a flexible stalk that allows Env tilting and variable exposure of neutralizing epitopes. Together, our results provide an integrative understanding of HIV assembly and structural variation in Env antigen presentation.

PubMed: 35123651
DOI: 10.1016/j.cell.2022.01.013

Experimental method

ELECTRON MICROSCOPY (9.1 Å)