7SKA

Sub-tomogram averaged structure of HIV-1 Envelope protein in native membrane

7SKA の概要

• エントリーDOI: 10.2210/pdb7ska/pdb
• EMDBエントリー: 25178 25186 25809
• 分子名称: Envelope glycoprotein gp120, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (13 entities in total)
• 機能のキーワード: hiv-1, envelope protein, glycoprotein, env, membrane bound, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 244801.51

構造登録者

Mangala Prasad, V.,Lee, K.K. (登録日: 2021-10-20, 公開日: 2022-03-09, 最終更新日: 2024-10-23)

主引用文献

Mangala Prasad, V.,Leaman, D.P.,Lovendahl, K.N.,Croft, J.T.,Benhaim, M.A.,Hodge, E.A.,Zwick, M.B.,Lee, K.K.
Cryo-ET of Env on intact HIV virions reveals structural variation and positioning on the Gag lattice.
Cell, 185:641-653.e17, 2022

PubMed Abstract: HIV-1 Env mediates viral entry into host cells and is the sole target for neutralizing antibodies. However, Env structure and organization in its native virion context has eluded detailed characterization. Here, we used cryo-electron tomography to analyze Env in mature and immature HIV-1 particles. Immature particles showed distinct Env positioning relative to the underlying Gag lattice, providing insights into long-standing questions about Env incorporation. A 9.1-Å sub-tomogram-averaged reconstruction of virion-bound Env in conjunction with structural mass spectrometry revealed unexpected features, including a variable central core of the gp41 subunit, heterogeneous glycosylation between protomers, and a flexible stalk that allows Env tilting and variable exposure of neutralizing epitopes. Together, our results provide an integrative understanding of HIV assembly and structural variation in Env antigen presentation.

PubMed: 35123651
DOI: 10.1016/j.cell.2022.01.013

実験手法

ELECTRON MICROSCOPY (9.1 Å)