7SD2

Murine Fab that recognizes Hev b 8 (profilin for Hevea brasiliensis)

Summary for 7SD2

• Entry DOI: 10.2210/pdb7sd2/pdb
• Descriptor: Heavy Chain Antibody IgE/Fab anti-profilin Hev b 8, Light Chain Antibody IgE/Fab anti-profilin Hev b 8 (2 entities in total)
• Functional Keywords: antibody, ige/fab fragment, immune system
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 6
• Total formula weight: 140526.99

Authors

Rodriguez-Romero, A.,Garcia-Ramirez, B. (deposition date: 2021-09-29, release date: 2022-08-10, Last modification date: 2024-10-16)

Primary citation

Garcia-Ramirez, B.,Mares-Mejia, I.,Rodriguez-Hernandez, A.,Cano-Sanchez, P.,Torres-Larios, A.,Ortega, E.,Rodriguez-Romero, A.
A native IgE in complex with profilin provides insights into allergen recognition and cross-reactivity.
Commun Biol, 5:748-748, 2022

PubMed Abstract: Allergies have become a rising health problem, where plentiful substances can trigger IgE-mediated allergies in humans. While profilins are considered minor allergens, these ubiquitous proteins are primary molecules involved in cross-reactivity and pollen-food allergy syndrome. Here we report the first crystal structures of murine Fab/IgE, with its chains naturally paired, in complex with the allergen profilin from Hevea brasiliensis (Hev b 8). The crystallographic models revealed that the IgE's six complementarity-determining regions (CDRs) interact with the allergen, comprising a rigid paratope-epitope surface of 926 Å, which includes an extensive network of interactions. Interestingly, we also observed previously unreported flexibility at Fab/IgE's elbow angle, which did not influence the shape of the paratope. The Fab/IgE exhibits a high affinity for Hev b 8, even when using 1 M NaCl in BLI experiments. Finally, based on the encouraging cross-reactivity assays using two mutants of the maize profilin (Zea m 12), this antibody could be a promising tool in IgE engineering for diagnosis and research applications.

PubMed: 35902770
DOI: 10.1038/s42003-022-03718-w

Experimental method

X-RAY DIFFRACTION (3.75 Å)